Whole Cell Patch Clamp Recording Performed on a Planar Glass Chip
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چکیده
منابع مشابه
Whole cell patch clamp recording performed on a planar glass chip.
The state of the art technology for the study of ion channels is the patch clamp technique. Ion channels mediate electrical current flow, have crucial roles in cellular physiology, and are important drug targets. The most popular (whole cell) variant of the technique detects the ensemble current over the entire cell membrane. Patch clamping is still a laborious process, requiring a skilled expe...
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Cristian Ionescu-Zanetti et al. have developed a technique for electrophysiological recording of whole mammalian cells on a microf luidic chip. The technique permits multiplexed recording of ion channel voltages, while leaving the cells visible for microscopic inspection and manipulation. The device records through many junctions along the microf luidic channel while a microscope observes from ...
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The patch clamp technique was first used by Neher and Sakmann (1976) to resolve currents through single acetylcholine-activated channels in cell-attached patches of membrane of frog skeletal muscle. The method they used (described by Neher, Sakmann & Steinbach, 1978) and subsequent refinements (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) have led to techniques for high resolution recording ...
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The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
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Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate depends on properties intrinsic to each channel protein as well as the mechanism by which it is generated and controlled and represents an important area of current research. Information about the operational dynamics of ion channel proteins can b...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2002
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(02)75646-4